The present invention relates to a trace amount of β-1,3- glucan, it can be detected how to manufacture it with the composition and the and β-1,3- glucan detection kit present in the sample. The compositions of the present invention the body fluid, or can be prepared by insects purifying the plasma and blood cell hemolysis water mixture of the sample by column chromatography, and, specifically, sufficient skill to chelating the calcium ions present in the sample and separation processes gained insect body fluid, or plasma and blood cell hemolysis water mixture samples in the presence rating claim, treated with calcium ions in the obtained sample to the sample and the separation process as a solvent or buffer containing sufficient chelating agent to the rating kill to obtain a fraction it can be produced by selecting a fraction showing a phenolic or oxy Days Days Pro phenol oxy-activating factor activity by β-1,3- glucan, under the calcium ion present in the resulting fractions. Using the composition according to the present invention, by extraction of the sample from the sample, and adding the composition and the calcium ions of the present invention in the sample, and measuring the phenol oxy Days or pro phenol oxy Days activating factor activity in the sample β a-1,3-glucan can be detected.
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