METHOD AND DETECTION PART FOR DETECTING INTERACTION SUCH AS HYBRIDIZATION, SUBSTRATE FOR BIOASSAY EQUIPPED WITH THE DETECTION PART, DEVICE FOR DETECTING INTERACTION SUCH AS HYBRIDIZATION, AND REAGENT KIT

摘要

PPROBLEM TO BE SOLVED: To realize (1) the prevention of nonspecific adsorption of a nucleic acid, an intercalator, etc. onto a solid phase surface in a reaction region, (2) the long-time maintenance of bonding characteristic of an intercalator used for detecting hybridization, and (3) the curtailment of a cleaning process in hybridization detection. PSOLUTION: This interaction detection method is used for storing or holding a salt-containing HEPES buffer solution S in the reaction region R. Nucleic acids (D, T) and an intercalator are prevented from nonspecifically adsorbing onto the electropositive solid phase surface 11a by adjusting the salt concentration of the buffer solution S. Further, this interaction detection part 1 is equipped at least with the reaction region R, a medium M stored or held in the reaction region R and containing the salt-containing buffer solution S. This substrate for bioassay including a DNA chip is equipped with the detection part 1. This device is used for detecting interaction such as hybridization. This reagent kit includes the salt-containing buffer solution, etc. PCOPYRIGHT: (C)2006,JPO&NCIPI