Method to Detect Differential Gene Expression in Cross-species Hybridization Experiments at Gene and Probe Level.

摘要

Whole genome microarrays are increasingly becoming the method of choice to study responses in model organisms to disease, stressors or other stimuli. However, whole genome sequences are available for only some model organisms, and there are still many species whose genome sequences are not yet available. Cross-species studies, where arrays developed for one species are used to study gene expression in a closely related species, have been used to address this gap, with some promising results. Current analytical methods have included filtration of some probes or genes which showed low hybridization activities. But consensus filtration schemes are still missing.