Novel lab-on-a-chip system for label-free detection of DNA hybridization and protein-protein interaction by surface plasmon resonance (SPR)

摘要

The detection of specific DNA sequences for the analysis of mutations as well as the detection of proteins gains increasing importance in the field of point-of-care diagnostics. Here, a novel low-cost lab-on-a-chip system for label-free detection of DNA hybridization and protein-protein interaction is introduced. The platform consists of a reader with disposable SPR chips produced by injection moulding. Micro optical elements are integrated into the chip to accomplish a simple connection to the optical reader. Automated, software-controlled reagent handling is achieved by a temperature-controlled microfluidic system comprising a syringe pump and a switching valve. The sensing area can be separated into maximum 40 parts for parallel analysis. Patterned functionalization is achieved by inverse micro contact printing. Several application examples, ranging from on-chip DNA hybridization up to the detection of antibodies inside diluted human blood serum, will be demonstrated.