Joining detection of the hybridization in with the device, and the nuclear acid and the target nuclear acid which for reagent kit null detection detect interaction such as substrate and the hybridization which for bioassay

摘要

Disclosed is a method for the detection of an interaction between substances. According to the method, a salt-containing HEPES buffer is allowed to exist in a reaction region that provides a place of interaction for the interaction. The concentration of the salt in the salt-containing HEPES buffer may be adjusted such that one or more substances each having a negative charge, such as a probe nucleic acid, target nucleic acid and/or intercalator, can be prevented from undergoing non-specific adsorption on a positively-charged surface of a solid phase. Also disclosed are an interaction detecting unit including the reaction region and a medium existing in the reaction region and including the salt-containing HEPES buffer; a bioassay plate including a DNA chip provided with a number of such interaction detecting units; a system for detecting an interaction such as hybridization; and a reagent kit including the salt-containing HEPES buffer.