Novel RNA interference induction vector, gene expression suppression method using the vector, and transformant into which the vector has been introduced

摘要

PPROBLEM TO BE SOLVED: To provide a new RNA interference-inducing vector easy to produce with higher gene expression-suppressive efficiency, and to provide a method for efficiently suppressing gene expression using the vector. PSOLUTION: The RNA interference-inducing vector is such as to be integrated with a sense chain sequence(Target2) encoding the genetic information of a gene and the corresponding antisense chain sequence(Target2') and have an inverted repeat base sequence structure, wherein tRNASPThr/SP1 as the promoter sequence of RNA polymeraseIII is integrated in the upstream region of the inverted repeat base sequence. With this vector, as gene expression-suppressive efficiency can be made higher, this vector can be utilized for gene function analysis. PCOPYRIGHT: (C)2004,JPO