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Adipocyte Insulin adpinsl with Insulin A and B chains and an effective method of treating type 2 diabetes in a subject using adipocyte insulin
Adipocyte Insulin adpinsl with Insulin A and B chains and an effective method of treating type 2 diabetes in a subject using adipocyte insulin
The present invention relates to an adipocyte Insulin adpInsl with Insulin A and B chains of SEQ ID Nos. 1 and 2, a effective method of treating type 2 diabetes in a subject using adipocyte insulin, said method comprising steps of administering the insulin to a diabetic intraperitoneally, an Insulin gene of SEQ ID No. 3, a process of isolating protein Insulin from the adipocytes of the Carp, said method comprising steps of reverse transcripting RNA of adipocytes to obtain cDNA, using oligonucleotide primers of SEQ ID Nos. 5 and 6 to identify AdpInsl gene from cDNA, and deducing amino acid sequence from cDNA to obtain protein Insulin, and an adipocyte of Catla Catla useful for producing the said Insulin.
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机译:本发明涉及具有SEQ ID Nos.1和2的胰岛素A和B链的脂肪细胞胰岛素adpIns1,一种使用脂肪细胞胰岛素治疗受试者的2型糖尿病的有效方法,所述方法包括将胰岛素给予糖尿病患者的步骤。腹膜内的SEQ ID No.3的胰岛素基因,是从鲤鱼的脂肪细胞中分离蛋白胰岛素的过程,所述方法包括使用SEQ ID No.5和6的寡核苷酸引物逆转录脂肪细胞的RNA以获得cDNA的步骤。从cDNA中鉴定AdpIns1基因,并从cDNA推导氨基酸序列以获得蛋白质胰岛素,以及可用于产生所述胰岛素的卡特彼勒卡特彼勒的脂肪细胞。
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