Process for immunoenzymatic test to be used in the detection of immunoglobulin G,M or the specific for proteins or glicolipideos purified the surface form of the parasite Toxoplasma gondii taquizoita and in the diagnosis of toxoplasmosis cru00c8nica, acute or Atarax

摘要

"Process for immunoenzymatic test to be used in the detection of immunoglobulin G,M or especu00ecficas to proteu00ecnas or glicolipu00ecdeos purified of superfu00eccie as taquizoita of the parasite Toxoplasma gondii and in the diagnosis of toxoplasmosis cru00c8nica, acute or eye.The present invention relates to test immunoenzimu00e1tico with proteins (AGS) or glycolipids (GIPL) purified from the surface of the form of the parasite T. gondii taquizoita.The test is called indirect, detects IgG antibodies specific for AGS, or IgM / IgA specific for GIPL, present in the sera of individuals or animals suffering from toxoplasmosis, acute, chronic.Carriers or not of ocular toxoplasmosis.The AGS or GIPL absorbed on solid support (microtu00e9cnica plates, tubes, spheres or papers of nitrocellulose or nylon) and placed to react (incubated with the sera tests.After incubation with the conjugate (anti immunoglobulin G, m, or the human enzyme) the reaction is revealed with a solution composed of the substrate of the enzyme used in conjugate (chromogen).Alternatively, the conjugate may be composed of anti imunoglobulu00edna g pork, sheep or feline associated with enzyme for the detection of animals with chronic toxoplasmosis.After the development of the reaction (formation of color) the same is paralyzed with acid solution and read visually (naked eye) or in a spectrophotometer with a specific filter for the colour formed.