IMMUNOENZIMACTIC TEST PROCEDURE TO BE USED FOR DETECTION OF G, M OR A SPECIFIC IMMUNOGLOBULINS FOR PURIFIED PROTEINS OR GLYCOLIPIDES OF THE SURGERY SURFACE OF THE TOXOPLASMA GONDII PARASITE AND THE DIAGNOSIS OF CHRONIC, ACUTE OR ACULAR TOXOPLASMOSIS

摘要

"Immunoenzymatic testing process to be used for the detection of immunoglobulins G, m or specific to purified proteins or glycolipids from the surface in the tachycazoite form of the parasite Toxoplasma gondii and in the diagnosis of acute or ocular chronic toxoplasmosis." This invention concerns the immunoenzymetic test with purified proteins (ags) or glycolipids (gipl) from the surface in the taquizoite form of the parasite t. Gondii. The indirect -denominated test detects IgG antibodies specific to AGS, or IgM / IgM specific to GIPL, present in sera from subjects or animals carrying acute, chronic toxoplasmosis,Holders or non -holders of ocular toxoplasmosis. The AGS or GIPL absorbed in solid support (microtechnical plates, tubes, spheres or papers of nitrocellulose or nylon) and placed to react (incubated) with the sera tests. After incubation with conjugate (anti -immunoglobulin g, m or human -enzyme) the reaction is revealed with a compound solution of the enzyme substrate used in the conjugate (chromogen). Alternatively, the conjugate may be composed of anti -immunoglobin g pig,Ovine or feline enzyme associated with the detection of animals carrying chronic toxoplasmosis. After the development of the reaction (color formation) it is paralyzed with acid solution and is visually read (by the naked eye) or spectrophotometer with a specific filter for the colour formed.