Method for the purification of heterologous polipeptu00ecdeo and broth or homogenate of fermentation.

摘要

Method for the purification of heterologous polipeptu00ecdeo and broth or homogenate fermentation.The present invention relates to a method for the purification of a heterologous polypeptide of interest from the broth or homogenate of microbial fermentation in which it is produced and the solubilized.This method involves the addition to the broth or homogenate effective quantity of a lactate solution 6.9 - diamino - 2 - etoxiacridina (ethacridine lactate) to precipitate the impurities of the host cell under conditions in which most of the polypeptide remains soluble and separation of the polypeptide desEjado the broth or homogenate. Is also described the broth or homogenate that contains the ethacridine lactate and polypeptide.