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Determining the ratio of prototype nucleotides to predetermined nucleotides, which is preferably greater than 1:1, by doping in walk-through mutagenesis
Determining the ratio of prototype nucleotides to predetermined nucleotides, which is preferably greater than 1:1, by doping in walk-through mutagenesis
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机译:通过在穿通诱变中掺杂来确定原型核苷酸与预定核苷酸的比例,该比例优选大于1:1
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摘要
A method of walk-through mutagenesis of a nucleic acid encoding a prototype polypeptide of interest is described. The method comprising selecting a predetermined amino acid and one or more target regions of the polypeptide, and synthesizing a mixture of oligonucleotides containing at each sequence position in the target region, either a prototype nucleotide that is required for synthesis of the prototype amino acid of the polypeptide, or a predetermined nucleotide that is required for synthesis of the predetermined amino acid, in which during the synthesis, the ratio of available prototype nucleotides, to available predetermined nucleotides, is greater than 1:1.
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