And 'doping' in the walk-through mutagenesis

摘要

It states concerning the method of inducing walk through mutation in the nuclear acid which the prototype polypeptide of the interest object the cord/code is done, but this method includes the stage which synthesizes the blend of origonukureochido which includes the specified nucleotide which is necessary for the synthesis of the prototype nucleotide or the specified amino acid which is necessary for the synthesis of the prototype amino acid of the polypeptide one of specified amino acid and the polypeptides or in each arrangement position of stage, and the target territory which select the plural target territories, here, the case of that synthesis ratio of the available prototype nucleotide and the available specified nucleotide exceeds the 1:1.