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method for the detection of nucleic acids of bacterial pathogens or parasites in the urine.

机译:尿中细菌性病原体或寄生虫核酸检测的方法。

摘要

The present invention relates to a method for diagnosing and/or monitoring a bacterial or parasitic infection by detection and quantification of the transrenal nucleic acids, derived from bacterial pathogenic agents or from parasites, in urine. The detection method optionally includes the isolation and the purification of the nucleic acids from urine by methods known in the art including pairing with molecular probes that are specific for the pathogenic agents, PCR hybridization, PCR, nested PCR, SSCP, LCR, and SDA. Diagnostic kits based on these detection methods are also claimed.
机译:本发明涉及一种通过检测和定量尿中来源于细菌致病因子或寄生虫的经肾核酸来诊断和/或监测细菌或寄生虫感染的方法。检测方法任选地包括通过本领域已知的方法从尿液中分离和纯化核酸,包括与对病原体特异的分子探针配对,PCR杂交,PCR,巢式PCR,SSCP,LCR和SDA。还要求保护基于这些检测方法的诊断试剂盒。

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