Promoter for functional characterization of protein-coupled g in the yeast Saccharomyces cerevisiae receptors.

摘要

Recombinant promoter fragment with the DNA sequence DNA compound. An object of the present invention was to identify another S. cerevisiae promoter which can be activated by the pheromone to and with which can achieve a strong regulated gene expression. Object of the invention is the promoter of the YNL 279 w gene of S. cerevisiae with SEQ ID NO. 4. It is also object of the invention a recombinant DNA fragment is the promoter of the YNL 279 w gene of Saccharomyces cerevisiae which, when operably linked with a structural gene, regulates transcription compound and has the DNA sequence SEQ ID NO. 4. The object of the invention a recombinant DNA fragment containing the promoter YNL 279 w a structural gene and wherein the promoter is functionally linked with the structural gene. The structural gene encoding for example a reporter gene or a protein to be produced in large quantities in S. cerevisiae. They are the subject of the invention a DNA vector and a recombinant S. cerevisiae cell containing such a recombinant DNA fragment. Such a recombinant S. cerevisiae cell is not expressed or preferentially expressed insufficient amounts of functionally activated Ste2p and Ste3p endogenous receptors.