Novel antioxidative compound isolated from the fruits of Catalpa ovata G. Don and isolation method thereof

摘要

The present invention relates to a novel antioxidants isolated from Catalpa ovata tree (Catalpa ovata G. D _ON) fruit and its separation method. More specifically, the nuts from Catalpa ovata C ₂₀ H ₂₀ O 7- oleic pinal 3,4' having the structure of _{6-dihydroxy-3} ', 5-dimethoxy-Plastic half (7-olefinal-3,4' naming a new active material with -dihydroxy-3 ', 5-dimethoxyflavane) was found.; The methanol extract of Catalpa ovata nut acetate to distribute the buffer solution of ethyl acetate and phosphoric acid to obtain the acetate soluble neutral fractions, the active fractions silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, octadecyl silsilran column chromatography , was finally remove the named material as a novel 7-oleic pinal 3,4'-dihydroxy-3 ', 5-dimethoxy-Plastic half having the structure C ₂₀ H ₂₀ O ₆ by steps such as HPLC , the structure of the material and determined by the MS, NMR analysis, was measured the antioxidant activity of the separation material.; 7-oleic pinal 3,4'-dihydroxy-3 ', 5-dimethoxy-Plastic half lead compound for a variety of active material not only can be used a new natural antioxidant having antioxidant effect of the present invention (lead compound ) as it will provide a wide range of applications, including in the availability.