6 FOSFOGLYUKONOLAKTONAZA FROM ESCHERICHIA COLI AND METHOD FOR PRODUCING L-AMINO ACIDS

摘要

1. 6 - u0444u043eu0441u0444u043eu0433u043bu044eu043au043eu043du043eu043bu0430u043au0442u043eu043du0430u0437u0430, u0432u044bu0431u0440u0430u043du043du0430u00a0 of group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of the following proteins.;(a) a protein comprising the amino acid sequence of the sequence number 2 (SEQ list id no: 2); and;(b) the protein consisting of u043eu0441u043bu0435u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u044c amino acids containing the u0434u0435u043bu0435u0446u0438u0438, substitution, insertion or addition of one or several amino acids in the sequence s u043du043eu043au0438u0441u043bu043eu0442 given in the list of sequences under the number 2 (SEQ id no: 2), which has at least 70% u0433u043eu043cu043eu043bu043eu0433u0438u0435u0439 towards u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du043eu0439 u043fu043eu0441u043bu0435u0434u043eu0432u0430u0442u0435u043bu044cu043d guests, the number 2 (SEQ id no: 2)and that has u0444u043eu0441u0444u043eu0433u043bu044eu043au043eu043du043eu043bu0430u043au0442u043eu043du0430u0437u044b activity 6.;2. a fragment of dna, encrypting u0444u043eu0441u0444u043eu0433u043bu044eu043au043eu043du043eu043bu0430u043au0442u043eu043du0430u0437u0443 6 to 1.;3. u0431u0430u043au0442u0435u0440u0438u00a0 - a producer of l - amino acids, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0, u0443u043au0430u0437u0430u043du043du0430u00a0 u0431u0430u043au0442u0435u0440u0438u00a0 modified so that the activity of 6 - u0444u043eu0441u0444u043eu0433u043bu044eu043au043eu043du043eu043bu0430u043au0442u043eu043du0430u0437u044b on 1 is increased.;4. u0431u0430u043au0442u0435u0440u0438u00a0 on p.3, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0, u0443u043au0430u0437u0430u043du043du0430u00a0 u0431u0430u043au0442u0435u0440u0438u00a0 belongs to the family of Enterobacteriaceae.;5. u0431u0430u043au0442u0435u0440u0438u00a0 on p.3, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0, u0443u043au0430u0437u0430u043du043du0430u00a0 6 u0444u043eu0441u0444u043eu0433u043bu044eu043au043eu043du043eu043bu0430u043au0442u043eu043du0430u0437u0430 obtained from bacteria belonging to the genus escherichia.;6. u0431u0430u043au0442u0435u0440u0438u00a0 on p.3, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0 what activity 6 - u0444u043eu0441u0444u043eu0433u043bu044eu043au043eu043du043eu043bu0430u043au0442u043eu043du0430u0437u044b enhanced by modifying the sequence controlling expression of gene 6 u0444u043eu0441u0444u043eu0433u043b u044eu043au043eu043du043eu043bu0430u043au0442u043eu043du0430u0437u044b on chromosome of the bacteria, so that the u044du043au0441u043fu0440u0435u0441u0441u0438u00a0 gene enhanced.;7. u0431u0430u043au0442u0435u0440u0438u00a0 for p.6, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0 because natural promoter of this gene has been replaced by a more powerful promoter.;8. u0431u0430u043au0442u0435u0440u0438u00a0 for p.6, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0, gene, encrypting the 6 u0444u043eu0441u0444u043eu0433u043bu044eu043au043eu043du043eu043bu0430u043au0442u043eu043du0430u0437u0443, selected from among a group of u0441u043eu0441u0442u043eu00a0u0449u0435u0439;(a) the dna sequence of the nucleotide u0432u043au043bu044eu0447u0430u044eu0449u0430u00a0 with 1 996, the list of sequence number 1 (SEQ id no: 1); and;(b) dna, u043au043eu0442u043eu0440u0430u00a0 u0433u0438u0431u0440u0438u0434u0438u0437u0443u0435u0442u0441u00a0 in rigid u0443u0441u043bu043eu0432u0438u00a0u0445 with nucleotide sequence with 1 996, the list of sequence number 1 (SEQ id no: 1), or with the u043eu043du0434u043eu043c, cooked by the sequence of nucleotides and encodes a protein with the activity of 6 - u0444u043eu0441u0444u043eu0433u043bu044eu043au043eu043du043eu043bu0430u043au0442u043eu043du0430u0437u044b.;9. u0431u0430u043au0442u0435u0440u0438u00a0 on p.8, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0, rigid u0443u0441u043bu043eu0432u0438u00a0u043cu0438 u00a0u0432u043bu00a0u044eu0442u0441u00a0 u0443u0441u043bu043eu0432u0438u00a0 in which money u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u0435u0442u0441u00a0 in 60u0441, u043au043eu043du0446u0435u043du0442u0440u0430u0446u0438u00a0 salt is 1xSSC and 0.1% sds, and the u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 launder all of 15 minutes.;10. u0431u0430u043au0442u0435u0440u0438u00a0 on p.3, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0, u0443u043au0430u0437u0430u043du043du0430u00a0 u0431u0430u043au0442u0435u0440u0438u00a0 modified in such a way that u044du043au0441u043fu0440u0435u0441u0441u0438u00a0 open framework u0441u0447u0438u0442u044bu0432u0430u043du0438u00a0 yddG strengthened.;11. u0431u0430u043au0442u0435u0440u0438u00a0 on p.3, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0 because the l - u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043eu0439 u00a0u0432u043bu00a0u0435u0442u0441u00a0 u0430u0440u043eu043cu0430u0442u0438u0447u0435u0441u043au0430u00a0 l - amino acid u0432u044bu0431u0440u0430u043du043du0430u00a0 of group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of l - tryptophan, l - phenylalanine a and l - u0442u0438u0440u043eu0437u0438u043du0430.;12. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 l - amino acids, including the stage of u0432u044bu0440u0430u0449u0438u0432u0430u043du0438u00a0 bacteria on p.3 in breeding environment, and u0432u044bu0434u0435u043bu0435u043du0438u00a0 from l - amino acids from u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 u0436u0438u0434u043au043eu0441 ti.;13. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 p.12, however, that the l - u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043eu0439 u00a0u0432u043bu00a0u0435u0442u0441u00a0 u0430u0440u043eu043cu0430u0442u0438u0447u0435u0441u043au0430u00a0 l - amino acid.;14. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 p.13, however, that the l - u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043eu0439 u00a0u0432u043bu00a0u0435u0442u0441u00a0 l - tryptophan.;15. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 p.13, however, that the l - u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043eu0439 u00a0u0432u043bu00a0u0435u0442u0441u00a0 l - phenylalanine.;16. way on any of the u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 question 14 or 15, so that the bacteria enhanced biosynthesis of aromatic amino acids u044du043au0441u043fu0440u0435u0441u0441u0438u00a0 genes.