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PHARMACOLOGICALLY ACTIVE COMPOUND OF THE NEW SLEEPING STATE DOWER-PHERON FOR MONITORING STRESSES AND AGING PROCESSES, METHOD FOR ITS ISOLATION AND DETERMINATION (CHARACTERIZATION)
PHARMACOLOGICALLY ACTIVE COMPOUND OF THE NEW SLEEPING STATE DOWER-PHERON FOR MONITORING STRESSES AND AGING PROCESSES, METHOD FOR ITS ISOLATION AND DETERMINATION (CHARACTERIZATION)
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机译:新的睡眠状态道夫-芬顿的药理活性化合物,用于监测应力和老化过程,其分离和确定(表征)的方法
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摘要
The present invention discloses a method for effectively isolating and purifying a physiological active dauer pheromone compound having the following structural formula I related to aging and stress of C. elegans, and determination of the structure of such material. First, ethanol extract is prepared by completely concentrating a liquid medium with ethanol after culturing C. elegans on a S. basal liquid medium, and then this ethanol fraction is further extracted with ethyl acetate. Then, impurities are removed from ethyl acetate extract by a silica-gel adsorption chromatography and a pheromone extract is isolated and purified by amine column chromatographic procedures using various solvents as eluent. Finally, a pheromone compound having structural formula I is completely isolated and characterized by size-exclusion HPLC.
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