OLIGONUCLEOTIDES, PRIMERS AND PROBES DESIGNATED FOR REALIZATION OF DETECTION METHOD AND/OR QUANTITATIVE DETERMINATION OF ADENOVIRUS NUCLEIC ACIDS WITH USING REAGENTS SET, METHOD FOR DIAGNOSIS AND METHOD FOR SELECTION

摘要

FIELD: virology, biotechnology, molecular biology.;SUBSTANCE: invention proposes oligonucleotides, primers and probes based on thereof designated for a detection method and/or quantitative determination of nucleic acids of adenoviruses in biological sample. Method involves amplification by polymerase chain reaction (PCR) in real time by using the proposed primers HEX1 and HEX2 wherein product is detected by using probe HEX. Also, invention proposes a set of reagents for realization of such method and methods for diagnosis and detection based on thereof. Proposed inventions provide carrying out detection of different serotypes of adenoviruses for a single reaction and quantitative determination of their small amounts.;EFFECT: improved methods for detection, diagnosis and selection.;7 tbl, 1 dwg, 8 ex