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Methods for ex vivo production of polyclonal and monoclonal antibodies without hybridomas and methods for producing immortalized cell populations
Methods for ex vivo production of polyclonal and monoclonal antibodies without hybridomas and methods for producing immortalized cell populations
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机译:没有杂交瘤的多克隆抗体和单克隆抗体的离体生产方法以及永生化细胞群的生产方法
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摘要
The invention includes methods and compositions for the generation of antibody-producing cells without the need for hybridoma formation. In one embodiment, the method includes the generation of monoclonal antibody producing cells by harvesting cells from a transgenic mouse with an immortalized cell population. In other embodiments, the method includes the use of a transgenic mouse with an immortalized cell population crossed with a transgenic mouse capable of producing humanized antibodies to produce a human and immortalized monoclonal antibody producing cell line.
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