METHODS FOR EX VIVO HYBRIDOMA-FREE PRODUCTION OF POLYCLONAL AND MONOCLONAL ANTIBODIES AND GENERATION OF IMMORTALIZED CELL POPULATIONS

摘要

The invention includes methods and compositions for the generation of antibody-producing cells without the need for hybridoma formation. In one embodiment, the method includes the generation of monoclonal antibody producing cells by harvesting cells from a transgenic mouse with an immortalized cell population. In other embodiments, the method includes the use of a transgenic mouse with an immortalized cell population crossed with a transgenic mouse capable of producing humanized antibodies to produce a human and immortalized monoclonal antibody producing cell line.