Plant cells transformed to express a recombinant polypeptide Insecticide, method for preparing the cell, Antibody Purified polypeptide and method of Detection of a set of elements (of immunodetection Kit)

摘要

Reveal strains of Bacillus thuringiensis crystal proteins comprising New have insecticidal activity against coleopteran insects including the Colorado Beetle larvae of the flour (Tribolium castaneum Beetle larvae) and Japanese (popillia tsunami CA). It also describes New Crystalline toxin genes of B.Thuringiensis, called cryet33 and Crystalline cryet34, encoding Proteins toxic to Coleoptera, Protein Crystal cryet33 (29 kDa), and cryet34 Gene encodes the Protein Crystal cryet34 14 kDa. Cryet33 cryet34 Crystalline proteins and are toxic to the larvae of the Colorado Beetle larvae of the flour and the Japanese Beetle.Also disclosed are methods of making and using transgenic Cell comprising the new sequences of nucleic acids of the present.