Polynucleotide, method for making a recombinant vector, recombinant vector, method for making a recombinant host cell, host cell, process for producing a polypeptide, polypeptide, antibody, antisense necleic acid molecule, method for producing transgenic plants, plant cells or plant tissue, plant cell, transgenic plant or plant tissues, methods for modulating acetyl coa synthesis in a host cell and acetyl-coa synthesis in a plant, plant tissue or plant cell, harvestable parts of the transgenic plant transgenic plant propagation material, method for producing fatty acids, carotenoids, (poly) saccharides, vitamins, isoprenids, lipids, waxy esters and / or polyhydroxyalkanoates, methods for identifying an agonist or antagonist of pno activity, compound, antibody, polynucleotide, vector, polypeptide, antibody, compound and a uses antisense nucleic acid molecule, pharmaceutical composition, kit, and method for producing a pharmaceutical composition

摘要

"Polynucleotide, METHOD FOR PRODUCING A RECOMBINANT VECTOR, RECOMBINANT VECTOR, METHOD FOR PRODUCING A host cell recombinant host cell, PROCESS FOR PRODUCING A polypeptide, polypeptide, antibody, Molecule Nucleic Acid ANTISENSE, METHOD FOR PRODUCING transgenic crops, CELLS PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT , TRANSGENIC PLANT PROPAGATION MATERIAL, METHOD FOR PRODUCING FATTY ACIDS, CAROTENOIDS, (POLY) SACARIDS, VITAMINS, LIPIDS, CERTEXY ANTERYTHONATE, AND / OR POLYHYDROXYANETIC POLYIDROIDS, POLYNUCLEOTIDE, VECTOR, POLYPEPTID, ANTIBODY, COMPOUND, AND A SPRING NUCLEIC ACID NUCLEIC ACID CELL, PHARMACEUTICAL COMPOSITION, KIT, AND METHOD TO PRODUCE A PHARMACEUTICAL COMPOSITION ". Polynucleotides encoding Pyruvate: NADP + oxidoreductases (PNO) as well as methods for obtaining them are provided. Furthermore, vectors comprising said polynucleotides are described, wherein the polynucleotides are operably linked to expression control sequences allowing expression in prokaryotic and / or eukaryotic host cells. In addition, polypeptides encoded by said polynucleotides, antibodies to said polypeptides and methods for their production are provided. In addition, methods for increasing the synthesis of acetyl CoA as well as methods for the production of fatty acids, carotenoids, isoprenids, vitamins, lipids, waxy esters, (poly) saccharides and / or polyhydroxyalkanoates or their metabolism products in particular are described. steroid hormones, prostaglandin, cholesterol, triacylglycerols, bile acids or ketone bodies, which comprise the expression of the polynucleotide or polypeptide described herein in a host cell or plant cell, plant tissue or plant. Methods for identifying compounds that are capable of activating or inhibiting PNO are also described. In addition, a pharmaceutical composition comprising the aforementioned inhibitor and antibody compounds is described. In addition, transgenic plants, plant tissues and plant cells containing the polynucleotides and vectors described above are described as well as the use of the mentioned polynucleotides, vectors, polypeptides, antibodies and / or compounds identified by the method of the invention in the production of acetyl metabolism products. CoA, for example, fatty acids, carotenoids, isoprenids, vitamins, lipids, (poly) saccharides, waxy esters and / or polyhydroxyalkanoates and / or their metabolism products, in particular steroid hormones, prostaglandin, cholesterol, triacylglycerols, bile acids and / or ketone bodies and pharmaceutical compositions.