TOOL FOR QUANTITATIVE REAL-TIME ANALYSIS OF VIRAL GENE EXPRESSION DYNAMICS IN SINGLE LIVING CELLS

摘要

Tool for quantitative real-time analysis of viral gene expression dynamics in single living cells The invention provides a method allowing the detection and the quantitative real-time measurement, at the single living cell level, of viral replication using a bioluminescent reporter gene and a digital light detection device sensitive to detect single photons with high efficiency and assign them a lateral x,y coordinate and precise temporal incidence (i.e. a "time point"), wherein the spatial and temporal characteristics of bioluminescence is indicative at the single living cell level of viral replication.