The nucleic acid detection cassette according to the present invention comprises a fluid keeping channels that may change the internal volume can be maintained and the reagent, is connected to the fluid held channel, fluid injection is open that can be made from the lateral side of the cassette 100 and the entry and exit ports to select a closing state in which the fluid injection is to be blocked, the fluid kept channel 111 is connected to the fluid feed and the open state can be made fluid transfer to said another fluid maintaining channel 111 the connection channel (116, 117) which can select a closing state, which may be blocked, and the entry and exit teller pads 404, 405 to maintain the port under the closed state, the closed state of the connecting channel (116, 117) and a connection pad 406, 407 can be maintained under.
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