DNA CHIP FOR SCREENING OF PEROXISOME PROLIFERATOR

摘要

A DNA chip and a kit for screening substances showing activity as a peroxisome proliferator are provided to be able to rapidly and accurately screen the peroxisome proliferator inducing abnormal cell reaction in vivo, thereby being widely applied to safety test of various materials. The DNA chip for screening of peroxisome proliferator comprises: a probe consisting of 121 to 587 nucleotides, capable of binding specifically to a gene whose expression is induced by a peroxisome proliferators; a linker including oligo (dT)15, -(CH2)6- and amine group in sequence, whose 3'-terminal of oligo (dT)15 is linked to 5'-terminal of the probe; and a solid substrate, whose surface is coated with aldehyde group, connecting with amine group of the linker via Schiff's base reaction. In the DNA chip, the probe is at least one gene selected from SEQ ID Nos: 1 to 12. The kit for screening of peroxisome proliferator comprises the DNA chip and a fluorescence-labeled deoxynucleotide to be used for labeling cDNA synthesized from RNA isolated from a sample. The method for screening peroxisome proliferator using the kit comprises the steps of: (a) administering a peroxisome proliferator candidate chemical to an animal; (b) isolating RNA from the animal; (c) synthesizing fluorescence-labeled cDNA from the isolated RNA using fluorescence-labeled deoxynucleotide in the kit; (d) applying the synthesized cDNA to a DNA chip for screening peroxisome proliferator in the kit to allow hybridization; and (e) after washing the hybridized DNA chip, examining the hybridized probe in the DNA chip by using a scanner.