DNA Chip for Screening of Peroxisome Proliferator

摘要

The present invention relates to a DNA chip which comprises probes hybridizable to genes expressed when individuals are dosed with peroxisome proliferator , a process for preparing the DNA chip, a screening kit comprising the said DNA chip, and a method for screening of peroxisome proliferator by using the said kit. The DNA chip for screening of peroxisome proliferator comprises: a probe of 121 to 587 nucleotides hybridizable to a gene expressed when individuals are dosed with peroxisome proliferator; a linker consisting of oligo (dT) ₁₅ , - (CH ₂ ) ₆ - and amine residue in a sequential order, whose 5 'end is bonded to the 3 'end of the said oligo (dT) ₁₅ ; and, a solid substrate attached to aldehyde residue which is bonded to the amine residue of the linker via Schiff's base reaction. The screening kit of the invention can be readily used for the selection of peroxisome proliferators which show peroxisome proliferating activity to induce abnormal cellular responses, which makes possible its practical application in the safety test of various materials.