CHIMERIC PROTEIN CONSISTING OF LTB AND HPV16 E6 PROTEIN AND THE USE THEREOF

摘要

A chimeric protein consisting of LTB(Escherichia coli heat-labile enterotoxin B subunit) and HPV16 E6(human papilloma virus type 16 E6) protein and use thereof are provided to induce an antibody specific to HPV16 E6 protein and mucosal immune response simultaneously, so that the chimeric protein is useful for production of a vaccine for preventing and treating cervical cancer. The chimeric protein consisting of LTB wherein the signal peptide region is deleted and HPV16 E6 protein has the amino acid sequence of SEQ ID NO:6 and is encoded by a gene having the nucleotide sequence of SEQ ID NO:5. A vector E6-LTB/pET29a contains the chimeric protein gene and is used for production of a transformed Escherichia coli BL21:E6-LTB/pET29a(KCTC 10893BP). The chimeric protein consisting of LTB and HPV16 E6 protein is produced by culturing the transformed Escherichia coli BL21:E6-LTB/pET29a(KCTC 10893BP), separating the chimeric protein from the cultured transformed microorganism and removing endotoxin from the separated chimeric protein.