CHIMERIC PROTEIN CONSISTING OF LTB AND HPV16 E7 PROTEIN AND THE USE THEREOF

摘要

A chimeric protein consisting of LTB(Escherichia coli heat-labile enterotoxin B subunit) and HPV16 E7(human papilloma virus type 16 E7) protein and use thereof are provided to produce a vaccine for preventing and treating cervical cancer capable of inducing an antibody specific to HPV16 E7 protein, and improve mucosa permeability of the vaccine by inducing mucosal immune response. The chimeric protein having the amino acid sequence of SEQ ID NO:6 consists of LTB wherein the signal peptide region is deleted, and HPV16 E6 protein. A vector E7-LTB/pET29a contains a recombinant gene encoding the chimeric protein having the nucleotide sequence of SEQ ID NO:5. The chimeric protein is produced by transforming Escherichia coli with the vector E7-LTB/pET29a to produce Escherichia coli BL21(DE3):E7-LTB/pET29a(KCTC 10895BP), culturing the Escherichia coli BL21(DE3):E7-LTB/pET29a(KCTC 10895BP), separating the chimeric protein from the cultured Escherichia coli, and removing endotoxin from the separated chimeric protein. An oral vaccine for preventing and treating cervical cancer comprises the chimeric protein as an effective ingredient.