CHIMERIC PROTEIN CONSISTING OF CTB AND HPV16 E7 PROTEIN AND THE USE THEREOF

摘要

A chimeric protein consisting of nontoxic CTB(cholera toxin B subunit) of Vibrio cholerae and HPV16 E7(human papilloma virus type 16 E7) protein and use thereof are provided to induce an antibody specific to HPV16 E7 and simultaneously induce mucosa immune response for promoting mucosa permeability of the antibody, so that the protein is useful as an effective ingredient of a vaccine for preventing and treating cervical cancer. The chimeric protein consisting of nontoxic CTB of Vibrio cholerae whose signal peptide region is deleted, and HPV16 E7 protein has the amino acid sequence of SEQ ID NO:6. A gene encoding the chimeric protein has the nucleotide sequence of SEQ ID NO:5. A method for producing the chimeric protein comprises the steps of: constructing a recombinant vector E7-CTB/pET29a containing the chimeric gene; transforming Escherichia coli with the vector to produce E. coli BL21(DE3):E7-CTB/pET29a(KCTC 10894BP); culturing the transformed E. coli; and separating the chimeric protein from the cultured E. coli, and purifying and removing endotoxin from the chimeric protein.