Method for detecting contamination and method for determining the detection threshold in genotyping screening

摘要

A method of detecting a contamination event by using a blank well and a replicate well occurring during a high-throughput screening is provided. In the method, a logistic regression equation for detecting a contamination in a genotyping experiment is determined, and a BWE (blank well error), an IRF (intraplate replicate failure) and an HWE (Hardy-Weinberg equilibrium) occurring in a blank well and a replicate well of a well plate during the genotyping experiment are checked. The contamination is detected based on a result value of the logistic regression equation, which is calculated by using the BWE, the IRF and the HWE as input variables of the logistic regression equation. Thus, the contamination can be precisely measured by the quantitative indexes without any qualitative analysis.