MEDIUM COMPOSITION FOR IN VITRO CULTURE OF BOVINE EMBRYO AND CULTURE METHOD USING THE SAME MEDIUM

摘要

The present invention relates to a culture medium composition for in vitro culture for improving bovine embryo development rate in a serum-free chemically defined medium, and an in vitro culture method using the same. More specifically, in order to increase the in vitro development efficiency of embryos in a serum-free confined medium during in vitro culture of in vitro fertilized embryos, myoinositol and epidermal growth factor are added to the serum-free medium To a defined medium and applying an appropriate two step culture according to the development stage to improve the in vitro culture efficiency and a medium composition used in the method. According to the present invention, by not using serum, it is possible to reduce the risk of disease transmission of an animal origin serum component and to improve the ambiguity of reading results by unknown factors included in the serum, The blastocyst production efficiency equivalent to that in using serum can be obtained.;Bovine embryo development, Embryo development rate, In vitro culture