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Culture medium for in vitro culture of in vitro-produced porcine embryo and method for in vitro production of porcine embryo using the culture medium

机译:用于体外培养的猪胚的体外培养的培养基和使用该培养基的猪胚的体外生产的方法

摘要

The invention provides an in vitro culture medium for in vitro-produced porcine embryo for the in vitro culture thereof, which can improve the quality of the resulting blastocyst and can raise the ratio of the development into fetus and infant after transfer, along with a method for in vitro culturing in vitro-produced porcine embryo using the culture medium, which can improve the quality of the resulting blastocyst and can develop the blastocyst into fetus and infant after transfer.;A culture medium for the in vitro culture of in vitro-produced porcine embryo, which contains lactic acid and pyruvic acid; a culture medium for the in vitro culture, which is conditioned with oviductal epithelial cell; and a method for in vitro culturing in vitro-produced porcine embryo, comprising culturing the in vitro-produced porcine embryo using the culture medium for the 0 to 2-day term after fertilization and subsequently culturing the embryo in a glucose-containing culture medium.
机译:本发明提供了一种用于体外产生的猪胚胎的体外培养基,用于其体外培养,其可以改善所得囊胚的质量,并可以提高转移后胎儿和婴儿的发育比例。用培养基体外培养体外生产的猪胚,可提高所得胚泡的质量,并能在转移后将胚泡发育成胎儿和婴儿。体外培养体外培养的培养基猪胚胎,含有乳酸和丙酮酸;以输卵管上皮细胞为条件的用于体外培养的培养基;本发明涉及一种体外培养猪胚的体外培养方法,其包括在受精后的0〜2天的条件下使用所述培养基培养体外猪胚,然后在含葡萄糖的培养基中进行培养。

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