The primer set for sensitive and specific detection of Xanthomonas oryzae pv. oryzae by Polymearse Chain Reaction

摘要

The present invention relates to a PCR primer capable of quickly and accurately diagnosing a specific DNA fragment of Xanthomonas oryzae pv. Oryzae from an infected organism, and to a method for detecting rice leaf blight pathogen using the same.;Primer of the present invention is a set of rice white leaf blight pathogen specific primer consisting of 20mer each can detect only this bacteria by PCR method. The primer set of the present invention is rice huinip diamond pathogen (Xanthomonas oryzae pv. Oryzae) only form a single band of 605bp and other janto Pseudomonas (Xanthomonas) of my similar species and other pathogenic strains has not detected a band DNA levels within 6 hours It is possible to quickly and accurately determine whether or not the infection of Xanthomonas oryzae pv. Oryzae is true and effective in predicting seed infection and development in cultivated field.;White leaf blight pathogen, Xanthomonas oryzae pv. oryzae, specific primers, diagnostics