The primer set for sensitive and specific detection of Xanthomonas axonopodis pv. glycines by Polymerase Chain Reaction

摘要

The present invention relates to a method for detecting a PCR primer, and water chestnut kongbul pathogens using the same which can diagnose quickly, accurately whether the infection from an organism infected with a specific DNA fragment of the diamond kongbul pathogen (Xanthomonas axonopodis pv. Glycines).;Primer of the present invention is a soybean blight pathogen-specific primer consisting of 23mer and 19mer can detect only this bacteria by PCR method. Primer set of the present invention is soybean fire pathogen ( Xanthomonasaxonopodis pv. glycines) only form a single band of 461bp and other janto Pseudomonas (Xanthomonas) of my similar species and other hospital type (Pathovar) has not detected a band quickly at the DNA level within 6 hours, exactly kongbul diamond pathogen (Xanthomonas axonopodis pv. Since it is possible to determine the authenticity of the infection of glycines , it is effective in predicting the seed infection and occurrence in the cultivation field.;Soybean blight, xanthomonas asonopodis pv. Glycine, specific primers, diagnostics