Determining method for genus level selection and population size of Lactic Acid Bacteria in Kimchi

摘要

A method for identifying lactic acid bacteria in kimchi in genus level and a method for determining the population size thereof are provided to analyze microbial flora in kimchi and migration of seed microorganism during fermentation, thereby producing kimchi having uniformed quality. The Lactobacillus sp. or Weissella sp., Pediococcus sp. or Leuconostoc sp., Weissella sp. or Leuconostoc sp., and Leuconostoc sp. are identified by performing PCR(polymerase chain reaction) for genome of a lactic acid bacterium isolated from kimchi by using a first primer set(SEQ ID NOs:1 and 2), a second primer set(SEQ ID NOs:3 and 4), a third primer set(SEQ ID NOs:5 and 6) and a fourth primer set(SEQ ID NOs:7 and 8), respectively. The each population size of Lactobacillus sp. or Weissella sp., Pediococcus sp. or Leuconostoc sp., Weissella sp. or Leuconostoc sp., and Leuconostoc sp. is determined by performing 15-25 cycles, 15-25 cycles, 15-35 cycles and 15-35 cycles PCR for genome of a lactic acid bacterium isolated from kimchi by using the first primer set(SEQ ID NOs:1 and 2), the second primer set(SEQ ID NOs:3 and 4), the third primer set(SEQ ID NOs:5 and 6) and the fourth primer set(SEQ ID NOs:7 and 8), respectively.