METHOD FOR PRODUCING ERYTHROCYTIC ANTIGEN FOR PREDICTING VIBRIOSIS IN FISH

摘要

FIELD: biotechnology, pisciculture.;SUBSTANCE: the innovation deals with preparing nutritive media, inoculation material and obtaining sensitin out of the strains Vibrio anguillarum N2 and VUR-19 followed by sensitization of ram's erythrocytes. Moreover, it is necessary to apply 20%-suspension of formalinized erythrocytes upon phosphate-buffer solution at pH being 7.0-7.4. Sensitization should be carried out upon water bath in two stages. Erythrocytes and sensitin should be taken at the ratio of 1:0.3-1:0.35.;EFFECT: higher efficiency.;2 ex