Genetic Marker for Discrimination and Detection of Vibriosis in fish and Method for Discrimination and Detection of Vibriosis in fish Using the Same

摘要

The present invention relates to a gene marker for discrimination and detection of Vibrio infectious disease, and a method for discriminating and detecting a causative bacterium using the same, and more specifically, selecting and amplifying a DNA sequence encoding a specific gene of the causative bacterium of fish Vibrio infectious disease Next, a peptide nucleic acid (PNA) that specifically recognizes the amplified product is hybridized, a temperature-dependent melting curve is obtained through temperature control of the hybridized product, and the obtained melting curve is analyzed to obtain a vibrio from the melting temperature. It relates to a method of determining an infectious disease or detecting whether or not a fish vibrio infectious disease is infected. The present invention enables accurate diagnosis within a few hours, thereby reducing economic loss through accurate and rapid diagnosis of Vibrio infectious diseases frequently occurring in aquaculture flatfish, and misuse of antibiotics through the use of appropriate antibiotics through accurate identification and early diagnosis And unnecessary increase in production cost.