Sample examination, involves bringing substance of sample in sample region to be detected in condition, and providing optical signal such that vertical wave with defined intensity-zero is formed in sample region to be detected

摘要

The process involves bringing a substance of a sample in a sample region to be detected in a condition. Another condition is induced by optical signals, and spatial limited partial regions within the sample region to be detected are omitted in a selective manner. The optical signal is provided such that a vertical wave (9) with defined intensity-zero is formed in the sample region to be detected, where the vertical wave is generated by focusing coherent light rays (10,11) in a pupil of an objective (13). The coherent light rays are provided by using glass fibers. An independent claim is also included for a microscope, in particular laser-raster-fluorescence microscope for spatial high resolution examination of samples.