METHOD FOR PRODUCING NEW PROTEIN BY UTILIZATION OF REPEATED ARRANGEMENT SUCH AS LEUCINE-RICH REPEATED (LRR) ARRANGEMENT, AND NEW PROTEIN OBTAINED BY THE METHOD AND GENE ENCODING THE NEW PROTEIN

摘要

PPROBLEM TO BE SOLVED: To provide a method for producing a new protein, by which many LRR arrangements existing in the natural world can effectively be used to efficiently and practically produce various proteins, to provide a new protein having ability corresponding to various pathogenic microbes existing in the natural world, and to provide a gene encoding the new protein. PSOLUTION: This method for producing the new protein is characterized by comprising a production step for producing a plurality of repeated arrangement units to contain leucine in the common domains on the amino acid sequence and/or base sequence information of a gene in the LRR domain of the gene having an LRR sequence, an amplification step for designing primers for the repeated sequence units, respectively, and obtaining repeated sequence unit genes by a PCR amplification, a polymerization step for randomly polymerizing the repeated sequence unit genes to produce the polymerization unit, an integration step for integrating the polymerization unit, a prescribed N-terminal cap unit and a prescribed C-terminal cap unit in a vector to produce a library. PCOPYRIGHT: (C)2008,JPO&INPIT