The protease secretion deficiency mutation body of the microbe the screening method of specifying

摘要

SolutionsAs for this invention, being specific method of protease secretion deficiency stocks of the microbe:Mutation body of Ciliata adding to gel;In queue/cue bait it does under the condition the mutation body of the Ciliata secreting the protein;Aforementioned the mutation body of the Ciliata is separated from the gel;Here, the Ciliata one secretion protease one substrate is contained at least at least in the aforementioned gel, and/or, aforementioned gel itself is the substrate, and/or, it can add the substrate afterwards, part spreads to the gel at least;At the same time, the protease activity which operated the substrate is measured, it regards method.