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Separation of Charged Solutes by Electrostatic Repulsion-Hydrophilic Interaction Chromatography
Separation of Charged Solutes by Electrostatic Repulsion-Hydrophilic Interaction Chromatography
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机译:静电排斥-亲水相互作用色谱法分离带电溶质
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摘要
In one aspect, a method of performing electrostatic repulsion-hydrophilic interaction chromatography on a protein, peptide, or amino acid includes providing a column having an anion-exchange material at a pH of less than about 4, and eluting the compound using a mobile phase comprising an amount of organic solvent sufficient to substantially balance the electrostatic repulsion of the stationary phase with hydrophilic interaction. In another aspect, a method of performing electrostatic repulsion-hydrophilic interaction chromatography on a nucleic acid or nucleotide comprises providing a column having a cation-exchange material at a pH of less than about 3.4, and eluting the compound using a mobile phase comprising organic solvent sufficient to substantially balance the electrostatic repulsion of the stationary phase with hydrophilic interaction.
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