A method for the detection and / or quantitative measurement of analyte comprising: (I) contacting (i) a conjugated enzyme specific for said analyte with (ii) antibody said analyte in the presence of an electrochemiluminescent detectant and enzyme substrate, wherein said enzyme converts said substrate into a product and wherein (a) a mixture of said substrate and said electrochemiluminescent detectant, or a conjugate of said substrate and said electrochemiluminescent detectant in the application of electrical energy emits electrochemiluminescence; and ( b) a mixture of said product and said electrochemiluminescent detectant, or a conjugate of said product and said electrochemiluminescent detectant, in the application of electrical energy emits electrochemiluminescence; and the electrochemiluminescence emitted by (a) differs from that emitted by (b); (II) applying electrical energy to said electrochemiluminescent detectant and (III) detecting or measuring electrochemiluminescence as an indication of whether or in what amount the analyte is present in the sample.
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