PURIFICATION OF LOW-ABUNDANT RECOMBINANT PROTEINS FROM CELL CULTURE

摘要

A method of purifying a fusion protein from a cell culture containing the same by inverse transition cycling (ITC), said fusion protein comprising a protein or peptide of interest coupled to a bioelastic polymer, is carried out by adding free bioelastic polymer as a co-aggregant to the cell culture solution so that the mass of fusion protein captured from the soluble cell lysate is increased.