DETECTION OF HYDROGEN PEROXIDE RELEASED BY ENZYME-CATALYZED OXIDATION OF AN ANALYTE

摘要

A diagnostic test kit for detecting hydrogen peroxide released by enzyme- catalyzed oxidation of an analyte within a test sample is provided. The test kit comprises a lateral flow device that contains a chromatographic medium, wherein the chromatographic medium defines a detection zone within which a chromogen is substantially non-diffusively immobilized in conjunction with an anionic compound. The chromogen is a leuco base, or a derivative thereof, which is capable of undergoing a detectable color change upon oxidation.