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ENVIRONMENTAL STRESS-RESPONDING PROMOTER, AND TISSUE-SPECIFIC GENE EXPRESSION METHOD USING THE SAME
ENVIRONMENTAL STRESS-RESPONDING PROMOTER, AND TISSUE-SPECIFIC GENE EXPRESSION METHOD USING THE SAME
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机译:响应环境的启动子以及使用相同方法的组织特异性基因表达方法
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摘要
A novel promoter having an environmental stress-responding property and capable of acting specifically in a specific tissue can be used to induce the expression of a gene specifically in the specific tissue. Specifically disclosed is a tissue-specific gene expression method comprising the steps of: providing a plant having a gene located downstream to an environmental stress-responding promoter containing DNA selected from the following items (a), (b) and (c): (a) DNA which comprises a nucleotide sequence selected from those depicted in SEQ ID NOs:1 to 8; (b) DNA which comprises a nucleotide sequence having the deletion, substitution or addition of one or more nucleotides in a nucleotide sequence selected from those depicted in SEQ ID NOs:1 to 8 and can act as an environmental stress-responding promoter; and (c) DNA which is capable of hybridizing with DNA comprising a nucleotide sequence selected from those depicted in SEQ ID NOs:1 to 8 under stringent conditions and can act as an environmental stress-responding promoter; and cultivating the plant under environmental stress conditions. The method enables to induce the expression of the gene located downstream to the environmental stress-responding promoter in a tissue-specific manner.
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机译:具有环境胁迫响应性质并且能够在特定组织中特异性起作用的新型启动子可以用于诱导基因在特定组织中特异性表达。具体公开了一种组织特异性基因表达方法,该方法包括以下步骤:提供一种植物,该植物的基因位于响应环境胁迫的启动子的下游,该启动子包含选自以下(a),(b)和(c)的DNA: a)DNA,其包含选自SEQ ID NO:1至8所示核苷酸序列的核苷酸序列; (b)DNA,其包含核苷酸序列,该核苷酸序列具有选自SEQ ID NO:1至8所示的核苷酸序列中的一个或多个核苷酸的缺失,取代或添加,并且可以作为环境胁迫响应启动子; (c)能够在严格条件下与包含选自SEQ ID NO:1至8所示核苷酸序列的DNA杂交的DNA,并且可以作为环境胁迫响应启动子;在环境压力条件下种植该植物。该方法能够以组织特异性方式诱导位于环境胁迫响应启动子下游的基因的表达。
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