METHOD FOR PREPARING HYDROXYLINOLEIC ACID USING LIPOXYGENASE FROM ZEA MAYS AND THE HYDROXYLINOLEIC ACID PREPARED BY THE SAME

摘要

Provided is a method for preparing hydroxylinoleic acid using maize (Zea mays) lipoxygenase and hydroxylinoleic acid prepared by the same. The method of the present invention comprises subjecting linoleic acid to dual regiospecific hydroperoxidation using a mixed solution containing linoleic acid and Zea mays lipoxygenase; reducing the resulting reaction product using triphenylphosphine or NaBH4 as a reducing agent; and separating the reduction product. The present invention enables production of hydroxylinoleic acid with controlled distribution of various hydroperoxylinoleic acids from linoleic acid, via the use of maize lipoxygenase which concurrently catalyzes 9-hydroperoxidation and 13-hydroperoxidation. Therefore, it is possible to provide various choices of biosynthetic pathways that can deal actively with a variety of stress applied to plants.