Regeneration of NADPH and/or NADH cofactors

摘要

The present invention discloses a method to regenerate NAD(P)H that comprises reacting together components comprising a phosphate donor, a substrate to be phosphorylated, an enzyme that catalyses both the liberation of phosphate from the phosphate donor and phosphorylation of the substrate, and an enzyme that catalyses dehydrogenation of the phosphorylated substrate and that uses NAD(P)+ as a cofactor such that NAD(P)H is formed.. The regeneration method may be coupled to any biosynthetic process using an enzyme requiring NAD(P)H as a cofactor.