2 617 Methods primers and kits for quantitative detection of JAK2 V617F mutants using pyrosequencing

摘要

A method for detecting a JAK2 V617F mutant is provided to easily, sensitively, accurately and quantitatively detect the JAK2 V617F mutant through pyrosequencing with good reproducibility and reduced time and cost, thereby being used for diagnosing, prognosing, and estimating the treatment progress of various lymphoblastic blood diseases such as chronic myeloid diseases, myeloproliferative diseases and myeloid metaplasia. A method for quantitatively detecting a JAK2 V617F mutant comprises the steps of: (a) subjecting a total DNA extracted by using a forward primer of SEQ ID : NO. 1 and a reverse primer of SEQ ID : NO. 4 to a PCR; and (b) subjecting the obtained PCR product to a pyrosequencing using a sequence analyzing primer of SEQ ID : NO. 5. A kit for quantitatively detecting a JAK2 V617F mutant comprises a forward and a reverse primers of PCR, respectively having a sequence of SEQ ID : NO. 1 and 4, for performing pyrosequencing, and a sequence analysis primer of the pyrosequencing having a sequence of SEQ ID : NO. 5.