METHOD FOR DETERMINATION OF ADIPOSE BODY DEVELOPMENT DEGREE IN BEES

摘要

FIELD: medicine; veterinary medicine.;SUBSTANCE: method is performed by incubating of bee's abdomen in formalin solution for 24 hours. The adipose body is prepared under microscope and immersed in fresh formalin fixing solution for 10 to 16 days; then it is transferred into KOH solution for 16 to 24 hours, and washed in distilled water. Smears are made from the cell suspension obtained, dried at room temperature, and stained with 1% methylene blue and hematoxylin-eosin. Cell nucleus width and length are measured; cell area to nucleus area rate is calculated, and adipose body development degree is evaluated.;EFFECT: invention provides accurate quantitative morphometric characteristics of adipose body cells.;2 dwg, 1 tbl