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Laccase enzyme and gene coding for this purpose.

机译:漆酶和为此目的编码的基因。

摘要

The invention relates to a new laccase enzyme, which can be isolated from the strains of the Melanocarpus genus, the M. albomyces strain in particular. The pH optimum of the enzyme is within 5-8 and the enzyme works at a temperature of 30-80 °C. The isoelectric point of the enzyme is about 4.0 as determined by isoelectric focusing and the molecular weight about 80 kDa, defined by SDS-PAGE. The enzyme is especially well suited to applications, wherein the pH and temperature conditions are high. The invention also relates to a gene that encodes laccase enzyme, and a laccase enzyme produced by recombinant technology.
机译:本发明涉及一种新的漆酶,其可以从黑果木属的菌株中分离,特别是从M.albomyces菌株中分离。该酶的最适pH在5-8之间,该酶在30-80°C的温度下工作。通过等电聚焦测定的酶的等电点为约4.0,通过SDS-PAGE确定的分子量为约80kDa。该酶特别适合pH和温度条件较高的应用。本发明还涉及编码漆酶的基因,以及通过重组技术产生的漆酶。

著录项

  • 公开/公告号DE60128091T2

    专利类型

  • 公开/公告日2008-06-12

    原文格式PDF

  • 申请/专利权人

    申请/专利号DE2001628091T

  • 发明设计人

    申请日2001-05-23

  • 分类号C12N9/02;

  • 国家 DE

  • 入库时间 2022-08-21 19:48:29

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